2021
Berkyurek, Ahmet C; Furlan, Giulia; Lampersberger, Lisa; Beltran, Toni; Weick, Eva-Maria; Nischwitz, Emily; Navarro, Isabela Cunha; Braukmann, Fabian; Akay, Alper; Price, Jonathan; Butter, Falk; Sarkies, Peter; Miska, Eric A
The RNA polymerase II subunit RPB-9 recruits the integrator complex to terminate Caenorhabditis elegans piRNA transcription Journal Article
In: EMBO J., vol. 40, no. 5, pp. e105565, 2021, ISSN: 0261-4189, (Publisher: EMBO).
Abstract | Links | BibTeX | Tags: integrator, piRNAs, RNA polymerase II, rpb-9, transcription termination
@article{berkyurek_rna_2021,
title = {The RNA polymerase II subunit RPB-9 recruits the integrator complex to terminate Caenorhabditis elegans piRNA transcription},
author = {Ahmet C Berkyurek and Giulia Furlan and Lisa Lampersberger and Toni Beltran and Eva-Maria Weick and Emily Nischwitz and Isabela Cunha Navarro and Fabian Braukmann and Alper Akay and Jonathan Price and Falk Butter and Peter Sarkies and Eric A Miska},
url = {http://dx.doi.org/10.15252/embj.2020105565},
doi = {10.15252/embj.2020105565},
issn = {0261-4189},
year = {2021},
date = {2021-03-01},
journal = {EMBO J.},
volume = {40},
number = {5},
pages = {e105565},
abstract = {PIWI-interacting RNAs (piRNAs) are genome-encoded small RNAs that regulate
germ cell development and maintain germline integrity in many animals.
Mature piRNAs engage Piwi Argonaute proteins to silence complementary
transcripts, including transposable elements and endogenous genes. piRNA
biogenesis mechanisms are diverse and remain poorly understood. Here, we
identify the RNA polymerase II (RNA Pol II) core subunit RPB-9 as required
for piRNA-mediated silencing in the nematode Caenorhabditis elegans. We
show that rpb-9 initiates heritable piRNA-mediated gene silencing at two
DNA transposon families and at a subset of somatic genes in the germline.
We provide genetic and biochemical evidence that RPB-9 is required for
piRNA biogenesis by recruiting the Integrator complex at piRNA genes,
hence promoting transcriptional termination. We conclude that, as a part
of its rapid evolution, the piRNA pathway has co-opted an ancient
machinery for high-fidelity transcription.},
note = {Publisher: EMBO},
keywords = {integrator, piRNAs, RNA polymerase II, rpb-9, transcription termination},
pubstate = {published},
tppubtype = {article}
}
PIWI-interacting RNAs (piRNAs) are genome-encoded small RNAs that regulate
germ cell development and maintain germline integrity in many animals.
Mature piRNAs engage Piwi Argonaute proteins to silence complementary
transcripts, including transposable elements and endogenous genes. piRNA
biogenesis mechanisms are diverse and remain poorly understood. Here, we
identify the RNA polymerase II (RNA Pol II) core subunit RPB-9 as required
for piRNA-mediated silencing in the nematode Caenorhabditis elegans. We
show that rpb-9 initiates heritable piRNA-mediated gene silencing at two
DNA transposon families and at a subset of somatic genes in the germline.
We provide genetic and biochemical evidence that RPB-9 is required for
piRNA biogenesis by recruiting the Integrator complex at piRNA genes,
hence promoting transcriptional termination. We conclude that, as a part
of its rapid evolution, the piRNA pathway has co-opted an ancient
machinery for high-fidelity transcription.
germ cell development and maintain germline integrity in many animals.
Mature piRNAs engage Piwi Argonaute proteins to silence complementary
transcripts, including transposable elements and endogenous genes. piRNA
biogenesis mechanisms are diverse and remain poorly understood. Here, we
identify the RNA polymerase II (RNA Pol II) core subunit RPB-9 as required
for piRNA-mediated silencing in the nematode Caenorhabditis elegans. We
show that rpb-9 initiates heritable piRNA-mediated gene silencing at two
DNA transposon families and at a subset of somatic genes in the germline.
We provide genetic and biochemical evidence that RPB-9 is required for
piRNA biogenesis by recruiting the Integrator complex at piRNA genes,
hence promoting transcriptional termination. We conclude that, as a part
of its rapid evolution, the piRNA pathway has co-opted an ancient
machinery for high-fidelity transcription.